When you install IIS AFTER .NET 2.0 framework, the rights of the ASPNET user had not been set correctly.
Resolution
Repair (Uninstall if repair does not work for you) .NET Framework 2.0
Simply run the following from command line to reset the IIS registry settings for aspnet user. Usually framework directory for .Net Framework 2.0 resides under %WindowsDir%\Microsoft.NET\Framework\v2.0.50727
%WindowsDir%\Microsoft.NET\Framework\v2.0.50727 > aspnet_regiis -i
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